Infertility

 

  • Background

    Overview

    • Definition: failure to conceive despite regular unprotected intercourse. WHO defines it as 1 year, NICE used to define it as 2 years (but now doesn't specify).
    • Investigate couples who are having difficulty after 1 year of trying.
    • 85% of people conceive within 1 year, and 92% within 2 years.
    • 50% are due to female causes, 30% male, and 20% unexplained. Sometimes both male and female causes are present.

    Female causes

    • Usually ovulatory – commonly PCOS – or tubal – commonly PID or endometriosis.
    • Age is the most important determinant of fertility. Declines steeply in late 30s.

    Ovulatory:

    • WHO class 1 (HPO failure → hypogonadotrophic hypogonadal): anorexia nervosa, intense exercise, Kallmann syndrome (congenital ↓GnRH, anosmia).
    • WHO class 2 (normogonadotrophic normoestrogenic): mainly PCOS, which causes 90% of anovulatory infertility.
    • WHO class 3 (primary ovarian failure → hypergonadotrophic hypoestrogenic): amenorrhea including premature menopause, radiation or chemotherapy, Turner's, fragile X, galactosaemia.
    • Hyperprolactinaemia, usually due to a prolactinoma, which inhibits GnRH release.

    Tubal:

    • PID is the commonest tubal cause. Chlamydia and gonorrhea cause 25%.
    • Adhesions: endometriosis, appendicitis, IBD, abdominal surgery.
    • Endometriosis can also cause tubal inflammation and distortion.

    Other:

    • Uterine abnormalities: fibroids, Asherman's syndrome (adhesions).
    • Cervical: mucus problems.
    • Irregular cycles e.g. >35 days or <23 days.

    Male causes

    • Unexplained low sperm count is the commonest male cause.
    • Drugs: alcohol, anabolic steroids, sulfasalazine.
    • Genetic: CF, Klinefelter's, Kartagener's (plus situs inversus).
    • Infection: previous mumps, chlamydia.
    • Anatomical: varicocele, cryptorchidism (undescended testes), retrograde ejaculation.
    • Trauma and surgery: hernia repair, vasectomy.
    • And ask about erectile dysfunction – some 'infertile' couples aren't even having sex.
  • History and examination

    • Most of the history should be done with both partners present, but make sure to check when each is alone if there's anything else they'd like to say.
    • Ask women the standard gynae history.
    • Ask men about any groin surgery, mumps, alcohol, and erectile dysfunction.
    • Ask about sex and relationship: frequency and timing, mood, technique.
    • Examine the female abdomen and pelvis.
  • Investigations

    Men

    Most male causes lead to detectable sperm problems:

    • Oligospermia is not enough sperm: may be secondary to hormonal problems or varicocele, but often idiopathic.
    • Azoospermia is no sperm. Non-obstructive causes include chromosomal causes. Obstructive causes include CF, infection, or vasectomy.
    • Asthenozoospermia is reduced sperm motility. Seen in Kartagener's.

    Normal ranges in semen analysis:

    • ≥1.5 ml
    • pH ≥7.2
    • ≥15 million/ml and ≥39 million per ejaculate.
    • Motility: ≥40% total or ≥32% progressive.
    • Vitality: ≥58% alive.
    • ≥4% normal morphology i.e. it is still normal for over 90% of sperm to be abnormal.

    Repeat semen analysis:

    • Immediately if azoospermia or severe oligozoospermia found.
    • After 3 months for any other abnormality.

    Further investigations if semen abnormal:

    • Endocrine: PRL, FSH (↑ in testicular failure), LH and free and total testosterone (↓ in hypogonadotrophism), estrogen, sex hormone-binding globulin.
    • Chromosomal and genetic testing.
    • Testes biopsy if there is severe oligospermia or azoospermia.
    • Antisperm antibodies not routinely recommended, as there is no treatment.

    Women

    First establish if ovulating. If cycles are regular and she has midcycle symptoms (fever, abdo pain), ovulation is likely to be occurring. Formal testing includes:

    • Mid-luteal progesterone, 7 days before period. >30 nmol/L = ovulation. If cycles are irregular, check sequential progesterone in luteal phase (every 2-3 days).
    • Day 2-5 gonadotrophins: ↑FSH suggests ovarian failure including premature menopause, ↑LH (specifically ↑LH/FSH ratio) suggests PCOS or menopause.

    Further endocrine testing:

    • ↑Testosterone in PCOS.
    • ↑Prolactin

    Transvaginal ultrasound:

    • Polycystic ovaries.
    • Endometriomas
    • Fibroids
    • Uterine polyps.

    Further tests for tubal disease:

    • Chlamydia: vaginal swab and PCR.
    • Investigate tubal patency and anatomy with hysterosalpingogram contrast XR (HSG) or laparoscopy plus dye. The latter is a gold standard, but more invasive.

    Not routinely recommended:

    • Cervical mucus testing: poor prediction of pregnancy success.
    • TFT, unless features of thyroid disease present.
  • Management

    Primary care

    Advise any couple concerned about fertility:

    • Sex every 2-3 days.
    • Stop smoking.
    • Get BMI under 30. This can cure anovulation in PCOS, and makes IVF more successful. Him too, as obesity → ↓sperm. If BMI under 19 in women, get it above this.
    • Folic acid.
    • Check rubella immune status and immunize if required.
    • Check smears up to date.

    After 1 year of trying, offer formal assessment, starting with:

    • Full history and female abdo/PV exam.
    • Testing: bloods to check for ovulation in women (if history unclear), semen analysis in men.
    • Referral to secondary care for further management and investigation.

    Indications for earlier referral:

    • Features of an underlying cause of infertility e.g. dysmenorrhea, pain, PMH of gynae problems.
    • Female >35 years old and trying for 6 months.

    Secondary care

    Female cause

    Class 2 anovulation (PCOS):

    • Lose weight.
    • Clomifene (1st line) and/or metformin (2nd line).
    • 3rd line: ovarian drilling – as the fragile-walled new vessels that re-grow release hormones more easily – or gonadotrophins.
    • Letrozole may be more effective, but use is currently off-label.

    Other ovulatory causes:

    • Class 1 anovulation (e.g. anorexia): gain weight, pulsed GnRH.
    • Class 3: IVF.
    • Bromocriptine – a dopamine agonist – if there is ↑PRL.

    Tubal disease:

    • IVF is best, but other options are worth trying as are often more available.
    • Resect or ablate any endometriosis.
    • Salpingostomy for distal blockage.
    • Adhesiolysis

    Male cause

    • Medical: gonadotrophins if hypogonadotrophism, bromocriptine if ↑PRL.
    • Intracytoplasmic sperm injection (ICSI) directly into egg if there is a motility problem.
    • If there is obstructive azoospermia: surgical correction of epididymal blockage is 1st line, and sperm retrieval from testis/epididymis is 2nd line.
    • Surgery for varicoceles does not improve fertility.

    Unexplained infertility

    If unable to conceive after 2 years of trying, despite normal investigations:

    • IVF
    • Intrauterine insemination (IUI) with ovarian stimulation (e.g. clomifene, gonadotrophins) is a cheaper alternative which may be more widely available.

    Other options

    IUI (without ovarian stimulation) is a useful alternative to sex for:

    • Those with difficulty having sex e.g. disabled, erectile dysfunction.
    • Those requiring sperm washing e.g. HIV +ve man.
    • Same-sex couples.

    Donor gametes:

    • Egg and sperm donors no longer have a right to anonymity: children can find out at 18 years old.
    • Egg sharing: a couple requiring eggs can pay some of the IVF costs of another couple, who will then give some of their eggs.
    • Both altruistic and named egg donations are allowed.
  • Clomifene

    Mechanism

    Estrogen receptor modulator → inhibits estradiol -ve feedback on hypothalamus → ovulation.

    Indications

    • Anovulation due to PCOS.
    • Not effective for women who are ovulating or with unexplained infertility.

    Side effects

    • Flushes
    • Visual
    • Abdo pain
    • ↑Risk of multiple pregnancies: 10% twins.
    • Ovarian cancer.
  • In vitro fertilisation (IVF)

    Background

    • 30% success per cycle if <35 years old, but only 5% if >40 years old.
    • Only available on NHS to those with a BMI <30 and no kids.
    • First screen couple for HIV, hep B, and hep C, to prevent transmission to fetus or woman.

    Procedure

    1. GnRH analogues used to suppress natural ovulation.
    2. Follicle growth stimulated with FSH, then hCG given to trigger ovulation.
    3. Oocyte retrieval via transvaginal ultrasound.
    4. Oocyte fertilised with sperm via IVF or intracytoplasmic sperm injection (ICSI).
    5. Oocytes cultured for 2-3 days, then graded.
    6. 2 embryos are transferred via catheter into the uterus.
    7. Pregnancy test 2 weeks later.

    Complications

    Ovarian hyperstimulation syndrome:

    • >20 ova form due to artificial ovulatory stimulation.
    • Presents a few days after hCG injection, with abdo pain/distention, nausea, and vomiting.
    • Manage with supportive care: analgesia, DVT prophylaxis.

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